With the addition of a PZ5 preamplifier, a suitable headstage and a commutator; electrophysiological recordings can be done in tandem with the fiber photometry and behavioral experiments.
The Fiber Photometry gizmo gives you runtime control of light driver parameters including driving frequency and light intensity. It supports multi-channel recordings in several brain areas of a subject or in different subjects. Raw signals, demodulated data streams and driver parameters for up to eight separate responses can be displayed at runtime and stored for further analysis. (See Fiber Photometry - RZ5P Processor How-To-Basics training video)
Easily view physiological responses to behavioral events in real-time or in post-analysis, using TDT’s Synapse software. Sensor input is demodulated using lock-in amplifier processing using Synapse’s ready-made Fiber Photometry gizmo. Select multiple driver/sensor combinations to extract the levels of specific responses of interest.
Setup the sensors, including calibration factor to normalize the output of two sensors with differing sensitivity, or to translate the output voltage to some metric (ie lumens, power)
Here is an example of what saturation of both the sensor, as well as the DACs looks like.
Here is an example of what normal operation might look like (except no actual physiological signal, so results show 0mv)
See the difference for neurophysiology experiment design, data collection and project management.
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