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Behavior + Fiber Photometry

Behavioral Control, Simplified

Description

Specs & Resources

TDT has two complete systems for behavioral control and fiber photometry:

iCon with RZ10x
The iCon controls your behavioral devices through TDT’s RZ10X processor for fiber photometry. The iCon is also hardware compatible with most third-party behavioral chambers.

iConZ with iX6
The iConZ also controls your behavioral devices, but the iConZ has a built-in processor – eliminating the need for an external TDT processor (RZ10x).  All of the processing power you need to control your behavioral paradigm is contained within the iConZ.  Adding the iX6 module to the iConZ provides a lower-cost system for fiber photometry. The iConZ is also hardware compatible with most third-party behavioral chambers.

Software
Both Fiber Photometry Systems are programmed in the same software that runs fiber photometry – Synapse.  The Pynapse Gizmo was developed for Synapse and is a coding environment for state-based behavioral tasks.

Simultaneously record fiber photometry and time-locked behavior with TDT.

Pynapse User Guide

iCon Behavioral Control Interface Manual

Synapse Manual

Fiber Photometry Manual

Intro to iCon and Pynapse: Part 1

Intro to iCon and Pynapse: Part 2

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System Components

RZ10X Lux-I/O Processor

iCon - Behavorial Chassis

Synapse - Neurophysiology Suite

Pynapse - Python Behavioral Control

WS Neuro Workstations

iConZ Behavioral Processor

iX6 - Fiber Photometry Module

LxM FP Optical Manifolds

Adding Behavior to the RZ10X:

The iCon plugs into the RZ10X and is an easy addition to existing TDT fiber photometry systems.

The iCon directly connects to third-party behavior chambers (including Med Associates and Harvard Apparatus) for easy control of food dispensers, lever presses, speakers, lickometers, and more.

Once connected, the Synapse software can simultaneously run fiber photometry and the iCon using the Pynapse Gizmo.

Behavioral Control with the iCon

The iCon is a simple-to-use behavioral control interface. The iCon has interchangeable modules to fit the needs of any experimental paradigm. Modules for the iCon include:

High Voltage Interface | iH10

For third-party devices requiring high voltage, such as food dispensers and lever presses

Multi-Function Interface | iM10

This analog I/O controls auditory speakers and microphones. The interface also has a touch sensor for lickometers

Other iCon modules are available for IR drivers and sensors (iR5) and digital logic (iL24).

Using the Pynapse Gizmo

Behavior and Fiber Photometry in Synapse

The Synapse software controls all aspects of event-based behavioral tasks and fiber photometry. The Pynapse Gizmo is a coding interface for behavior control for Synapse.

Pynapse makes it easy to program event-based behavior and drive the iCon. Encoded states will change based on subject inputs via the iCon, other event outcomes, or timers. Information in Pynapse is easily accessible from MATLAB and Excel, and all data is saved into one log file for easy retrieval.

Use Synapse and Pynapse to run self-administration and fear-conditioning tasks.

RZ10X for Fiber Photometry

TDT’s Fiber Photometry is an out-of-the-box solution designed for researchers. Our user-friendly Synapse software provides a seamless interface to the real-time LUX RZ10X processor.

With Synapse, record and view your fluorophore responses in real-time while behavior and video data is automatically time-locked. TDT allows you to focus on the research, not the equipment.